Application Note

Purification Of An Acidic IgM For Large Biomolecule Purification

Source: Bio-Rad Laboratories, Inc.

By Jamie C Greenwood II, Payal Khandelwal, William H Rushton, Carsten Voss, Bio-Rad Laboratories

Molecule

IgM antibodies are one of the fastest growing groups of diagnostic and therapeutic candidates. The large size of IgMs makes their purification very challenging. Bio-Rad has developed a new strong anion exchange resin (AEX), Nuvia HP-Q, to overcome the multiple issues faced when purifying large biomolecules. Purification of an acidic IgM was performed using Nuvia HP-Q Resin, which resulted in good binding and purification. Further polishing of the Nuvia HP-Q eluate pool on the mixed-mode CHT Ceramic Hydroxyapatite Media led to highly pure IgM, with minimal product- and process-related impurities. The workflow resulted in >99% IgM purity, as measured by size exclusion chromatography (SEC) and SDS-PAGE analysis. This AEX-based purification platform helps overcome the limitations of traditional ion exchange resins for IgM purification and provides a successful and scalable platform strategy. The purified IgM is suitable for diagnostic applications and potentially for pharmaceutical applications.

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