Rapid, Serum-Free, High-Yield Antibody Production In Nunc PETG Roller Bottles Application Note

Monoclonal and polyclonal antibodies have been an essential tool for research for well over two decades. more recently, antibody-based therapy has emerged as an important approach for the treatment of a number of diseases. In this report, we describe a comprehensive system for antibody production using Thermo Scientific™ Nunc™ PETG Roller Bottles. This serum-free system (TIB-105) allowed the expansion of a hybridoma cell line growing in a single T-flask to a maximum cell density of ~1.2 x 10⁶ cells/mL in 8 days, with a peak antibody concentration of 230 μg/mL by 17 days, yielding about 184 mg per 800 mL roller bottle. By harvesting cells at peak viable cell count and subpassaging 1 roller bottle to 10 additional roller bottles, virtually unlimited scalability would be possible with no change in the peak cell density or peak antibody concentration in subsequent passages.  These data suggest that by the 4th passage, the system could produce more than 18 g of antibody from a single T-80 flask within 21–24 days.