Newsletter | May 22, 2020

05.22.20 -- Top Considerations And Troubleshooting Tips For Protein Analysis

 
How Buffer pH And NaCl Affect Size Exclusion Chromatography
 

Size exclusion chromatography (SEC) should separate proteins only according to their hydrodynamic radius (size). Using a design of experiments approach, this study describes the effect of buffer pH and NaCl concentration on proteins purified by SEC.

Quality Matters: SEC Analysis For Antibody Aggregates
 

Size exclusion chromatography (SEC) is the go-to analytical method in the analysis of therapeutic protein drugs. This article describes the factors affecting the reproducibility of columns and how you can minimize column variation.

Fundamentals About Analytical SEC
 

Size exclusion chromatography (SEC) is currently the most powerful chromatography technique for obtaining reliable information about the size of biomolecules under native conditions. This article presents fundamentals about analytical SEC and describes how new-generation agarose-based SEC columns are used in analysis of biomolecules.

Master Analytical SEC Running Conditions
 

Need to find out how to calibrate your size exclusion chromatography (SEC) column? Or how different additives affect your results? This article presents useful insights in response to these questions.

Unexpected SEC Results: Can It Be Improved?
 

Sometimes your size exclusion chromatography (SEC) results don’t look like you want them to. Fix issues such as poor resolution, peak tailing, and fronting with the tips outlined in this article.