AAV Process Intensification Using High Salt Lysis And Salt Tolerant Endonuclease

The production of adeno-associated virus (AAV) vectors involves a critical midstream step combining cell lysis and nuclease treatment to ensure patient safety and enhance downstream process efficiency. Addressing the challenges of cell lysis, this paper explores the use of detergents and their impact, considering regulatory restrictions and requirements. Additionally, it investigates the effect of high salt concentrations on AAV yield and infectivity during the midstream steps, offering insights into improved production methods.

Moreover, the paper discusses the development and application of a salt-tolerant endonuclease, Benzonase^® Salt Tolerant, addressing the historical limitations of conventional endonucleases in high salt conditions. This advancement allows for enhanced AAV production, providing higher titers and infectivity while maintaining patient safety and process integrity.