Alkaline Stability Of Modern Protein A Chromatography Resins

Protein A affinity chromatography is the major purification technique for the capture of antibodies or Fc-fusion proteins and enables a platform approach to downstream processing. Recent years have seen several modern protein A resins with high binding capacity, which also claim alkali resistance up to 0.5 M NaOH and more for cost-effective cleaning. But are all these protein A resins equally alkali resistant and how does that impact resin lifetime and purification performance?

Here, we share findings from a resin lifetime study that was performed over 150 cycles, using clarified cell harvest in every cycle, to evaluate the alkaline stability of three protein A-based resins — MabSelect PrismA™ (Cytiva), Praesto™ Jetted A50 (Purolite Corp.), and Amsphere™ A3 (JSR Corp.).