Capto Core 700: Chromatography Medium

Capto Core 700 is a multimodal chromatography medium that is designed for flowthrough purification of large proteins or viruses in vaccine processes. The performance of the medium can be compared with gel filtration chromatography, but Capto Core 700 offers improved productivity and process economy. The multimodal property of the ligand in the core ensures strong binding with most impurities over a wide range of pH and salt concentration.

Capto Core 700 enables:

• Higher sample loads
• Higher flow rates
• Simple optimization and scale-up

The Capto Core 700 beads consist of a ligand-activated core and inert shell. The core bead design of this medium gives dualfunctional properties that make it possible to combine size separation with binding chromatography. The pore size of the beads gives a restricted access to the ligand-activated core and provides a cut-off for molecules larger than Mr 700 000. Relative to size-exclusion chromatography, Capto Core 700 provides the advantages of high purity, improved process economy, and increased productivity by:

• Enabling significantly higher sample load
• Enabling higher flow rates
• Ensuring straightforward optimization and scale-up

The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of the various contaminants small enough to enter into the core. The multimodal property of the ligands in the core ensures strong binding with most impurities over a wide range of pH and salt concentrations.