Capto L is an affinity chromatography medium for the process-scale capture of a broad range of antibodies and antibody fragments.
Capto L is an affinity chromatography medium for the process-scale capture of a broad range of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding protein L ligand. Capto L is therefore suitable for capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (dAbs, in addition to binding all classes of Ig (IgG, IgM, IgA, IgE and IgD).
Key performance characteristics of Capto L include:
Purification conditions are broadly similar for many antibody fragments containing the kappa light chain, and can easily be modified to match the specific properties of individual target molecules. This will result in significant savings in costs, effort and time during process development. Gains can also be won in manufacturing since the high selectivity of affinity capture will help reduce number of subsequent polishing steps as compared to other, less selective purification media.