Capture Of Histidine-Tagged Molecules Using Biacore His Capture Kit

His Capture Kit is suitable for use with carboxyl-derivatized Biacore™ sensor chips and Series S sensor chips of the following series: Sensor Chip CM3, Sensor Chip CM4, Sensor Chip CM5, Sensor Chip C1, or Sensor Chip PEG.

Review this procedure to find recommendations for immobilization of histidine-tagged molecules (ligands) by capture to an anti-histidine antibody covalently coupled to a sensor chip surface.