Codetection Of Viral Pathogen RNA And DNA Using One-Step Multiplex RT-qPCR

By Jason Ma, Michelle Kong, and Steve Okino

RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics, as well as food safety. There are multiple benefits of this technology: unparalleled sensitivity, quick time to results, simultaneous detection of multiple targets, scalable throughput, and low cost. However, when dealing with the challenges of pathogen detection, not all RT-qPCR reagents are created equal. In this application note, we demonstrate a sensitive codetection of viral RNA and DNA targets in a multiplex setting using a novel one-step multiplex RT-qPCR supermix.