Demonstrating The Scalability Of Cytiva P-Grade Depth Filters For CHO Cell Culture Clarification

By Rajeshwar Chinnawar, Bridget Marriott, Luke McCarney, and Nick Marchand

Clarification using depth filtration is a standard practice in monoclonal antibody (mAb) manufacturing, serving as the initial step to eliminate cells and prepare cell culture harvest for downstream chromatography and purification processes. This step is crucial as it significantly affects product recovery and the efficiency of subsequent purification stages. Industry standards for evaluating depth filtration processes and filters typically include metrics such as turbidity reduction and filter capacity or lifespan.

Here, we showcase the scalability of Stax™ mAx depth filters, demonstrating their effectiveness from lab-scale capsules to manufacturing-scale capsules. The performance of these filters remains consistently high across various scales, as evidenced by metrics including filtration throughput, turbidity reduction, impurity removal, mAb yields, and particle counts.