Is your protein unable to bind efficiently to your resin? If you are performing flow-through (FT) purification, monomer recovery could be poor in the flow-through fraction due to nonoptimal conditions, a nonideal resin, or both.
Performing a design of experiment (DOE) study of different pH, salt concentrations, and resins will help you select the best conditions and resin for your purification success.
A DOE study showed that Nuvia aPrime 4A Resin offers better monomer recovery than both Capto adhere Media for the purification of a basic protein (approx. pl of 8.4) at the optimal FT operation pH of 6.5.
Click Here To View Detailed Technical Features of the Nuvia aPrime 4A Resin