How To Improve Lentivirus Polishing Recovery Using Capto™ Core 700 Resin

By Åsa Hagner-McWhirter, Brigitta Németh, Nils Norrman, Ola Lind, Mikaela Löfbacka, Lisa Marx, Greta Hulting, Josefin Thelander, Johanna Viiliäinen, Ann-Christin Magnusson, and Jean-Luc Maloisel

One of the most efficient gene transfer vectors is lentiviruses, enveloped RNA viruses belonging to the retroviridae family. Lentiviruses integrate their RNA into the host cell DNA and are commonly used for chimeric antigen receptor (CAR) T-cell therapy. The purification of lentiviral vectors is very challenging due to the low stability of this enveloped virus. Throughout the bioprocess, from harvest to final sterile filtration, efforts must be made to reduce conditions that can negatively impact a good physical and infectious titer recovery. In this study, we used capture with weak anion exchange and optimized the Capto™ Core 700 resin polishing step to maximize infectious recovery.