Datasheet | November 3, 2025

HyClone™ Peak Expression Medium

Source: Cytiva
HyClone Peak Expression Medium

HyClone™ peak expression medium is a chemically defined, animal-derived component-free solution designed to optimize AAV production in HEK293 cell lines. Its hydrolysate-free formulation supports high transfection efficiency, consistent cell growth, and robust viral titers, which makes it ideal for both research and manufacturing environments. With stable L-glutamine and poloxamer 188 included, the medium enables direct adaptation and seamless transition from growth to production phases. Performance comparisons show improved viability and higher peak cell density over alternative media across multiple HEK293 lines. Available in both liquid and powder formats, it’s compatible with PEI and other lipid-based transfection reagents, supporting scalability from small to large-scale applications.

For teams focused on maximizing yield and consistency in viral vector production, discover how this medium offers a reliable and regulatory-friendly solution.

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