High-Density Culture Strategies For Improved Scalability Application Note

High-density culture (>40 x 106 cells/mL) strategies reduce processing time and include perfusion, concentrated fed-batch, and intensified seed trains—whereas an N-1 perfusion bioreactor is used to seed a production bioreactor. In this study, the integration of the Thermo Scientific™ HyPerforma™ Single-Use Bioreactor Turnkey (S.U.B. TK) and the perfusion XCell™ ATF 6 system was used to achieve high-density cultures, without modifications to standard components. Integration of the HyPerforma S.U.B. TK and the ATF 6 system is capable of being used for a high-density seed train or as a production vessel system. Scalability criteria were also generated using standard components.