Mild pH Protein A Purification Step With MabSelect™ Mild Elution Resin

As antibody modalities expand beyond traditional IgGs, purification strategies are being pushed to adapt. Many newer formats, including bispecific antibodies, IgG4s, and Fc fusion proteins, are particularly sensitive to the low-pH conditions commonly used during protein A elution. Exposure to pH 3.5–4.0 can trigger aggregation, reducing monomeric purity and yield while increasing the burden on downstream polishing steps. This has driven a shift away from one-size-fits-all capture toward more flexible, challenge-specific resin selection. By enabling antibody capture at milder elution conditions, protein A resins designed for higher pH elution offer a way to protect vulnerable molecules without sacrificing performance. Delve into the data to see how elution around pH 5.0 can help maintain product quality during capture, offering a practical option for teams working with aggregation-prone antibody formats.

Access the full application note to explore how milder elution can reshape your capture strategy.