Article | February 15, 2023

mRNA Product Purification Process Strategies

Source: Cytiva
Feature Image 1

Explore the mRNA universe with Cytiva. In this series, Cytiva scientists share their journey and insights on mRNA synthesis for research purposes and they share their advice on how to scale.

Impurities from in vitro transcription (IVT) – including DNA template, enzymes, NTPs, salts, double-stranded (ds) RNA, and truncated mRNA – can induce undesired immune responses and negatively impact translation efficiency. For these reasons, the mRNA must be purified. There are several options to purify mRNA: lithium chloride (LiCl) precipitation; oligo deoxythymidine (dT) magnetic particles; and chromatography. When designing a purification process, consider scalability and suitability for a manufacturing environment.

Discover the pros and cons of each option, the challenges and strategies for the sterile filtration of mRNA, and more.

access the Article!

Get unlimited access to:

Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue. X

Enter your credentials below to log in. Not yet a member of Biosimilar Development? Subscribe today.

Subscribe to Biosimilar Development X

Please enter your email address and create a password to access the full content, Or log in to your account to continue.

or

Subscribe to Biosimilar Development