Development of upstream processes, that maximize productivity without increasing complexity, relies on the right combination of medium and feed and the ability to maintain tight control of culture conditions. In fed-batch mode, cells are initially grown in a relatively lean production medium followed by regular feed supplementation to achieve the desired product yield.
Cellvento® ModiFeed Prime COMP is a chemically-defined (CD), highly concentrated, single part, pHneutral feed for replenishing depleted nutrients required for cellular function and to extend the production phase in fed-batch mode. It is formulated for fed-batch production of monoclonal antibodies (mAbs) and next-generation biopharmaceuticals in all Chinese Hamster Ovary (CHO) cell-based expression systems, in combination with Cellvento® 4CHO COMP and EX-CELL® Advanced CHO production media, or many leading base media for CHO cells.
This application note we presents an optimized feeding strategy and demonstrates how this easy-to-hydrate, single-part, pH neutral feed can support optimal cell growth and high productivity of CHO cell lines. It describes performance and scalability of the highly-concentrated, compacted feed and shows how it can help reduce workflow complexity and risk to the process.