Performing dilution, concentration, and washing steps in a closed and automated manner is important in GMP cell therapy manufacturing. To assist in this process, Cytiva offers the CultureWash C-Pro application — a flexible, closed, and automated cell washing procedure — for use on the Sepax C-Pro cell processing system. Using a model T-cell line, we demonstrate the impact of varying rinse volume, intermediate volume, and manual extraction parameters on overall cell recovery with a final product dose volume of 20 mL. In this study, > 86% recovery was achieved, with 95% average recovery when manual extraction was enabled. We also investigate compartments where cell loss occurred and discuss strategies to maximize cell recovery.