Poster Review Non–Affinity Based Purification Platform for Neutral/Basic IgMs

Source: Bio-Rad Laboratories, Inc.

Speaker : Dr. Carsten Voss, Application Specialist, Process Chromatography

Dr. Voss reviews the biological properties of IgM antibodies that make them very effective vehicles for in vitro diagnostics and therapeutics. The purification of IgM antibodies is far more complex than that for IgG antibodies. Furthermore, affinity chromatography is not ideal for purifying IgMs due to the required elution conditions. In this poster review, Dr. Voss discusses the non–affinity based platform for IgM purification of neutral and basic IgMs. This strategy utilizes a cation exchange (CEX) resin, Nuvia™ S Resin, for capture and a mixed-mode media, CHT™ Ceramic Hydroxyapatite Media, for polish purification. The protocol was optimized with three different IgMs and present reducing and nonreducing PAGE images of the purified samples. This strategy is simple, scalable, and efficient and thus well suited for purification of diagnostic IgMs.