Seed Train Intensification Using High Cell Density Cryopreservation And Specially-Designed Expansion Medium

By Mona Bausch, Melanie Brandl, Ph.D., and Habib Horry, Ph.D.

Strategies such as process intensification have the potential to create shorter lead times, reduce plant footprint, increase flexibility and reduce COGs. Upstream processes can be intensified using a number of strategies which can deliver significant improvements in speed, flexibility and reliability. In this whitepaper, we explore two of these approaches – High cell density cryopreservation (HCDC) and the use of a perfused seed train including a specially-designed expansion medium.