By Ratish Krishnan, MilliporeSigma
The ultimate goal of clarification is to prepare your cell culture feed stream for downstream chromatography and purification. The race for commercialization of gene therapies has provided an opportunity for exploring platform and templated processes in viral vector bioprocessing. Depth filtration and Benzonase® endonuclease digestion are considered gold standard unit operations in Adeno Associated Virus (AAV) production processes. Optimization of this midstream set of unit operations use should be considered a mandatory and often crucial step in process development. In this poster, we explore data driven strategies for clarifying harvest feed streams.