Strategies For Clarifying Harvest Feed Streams In Viral Vector Bioprocessing

By Ratish Krishnan, MilliporeSigma

The ultimate goal of clarification is to prepare the cell culture feed stream for downstream chromatography and purification. The race for commercialization of gene therapies has driven the need to explore platform and templated processes in viral vector bioprocessing. Depth filtration and Benzonase® endonuclease digestion are considered gold standard unit operations in Adeno Associated Virus (AAV) production processes. Optimization of this midstream set of unit operations should be considered a mandatory and often crucial step in process development. In this poster, we explore data driven strategies for clarifying harvest feed streams.