Two-Step Purification Of Monoclonal IgG1 From CHO Cell Culture Supernatant
The increasing demand for monoclonal antibodies (MAb) as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the demand for more effi cient purifi cation processes has increased. This application note describes a two-step process for MAb purifi cation based on MabSelect SuRe™ and Capto™ adhere, a strong multimodal anion exchanger.
The two-step process is applied to the purifi cation of monoclonal IgG1 antibodies from CHO cell culture supernatant and the results are compared with data from a process based on a three-step process. If necessary, Capto adhere can be used as a second or third polishing step in any MAb purifi cation platform.
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