Upstream Process Intensification With Dynamic Perfusion

Perfusion has long promised better yield and product quality than fed-batch for monoclonal antibody production, yet its complexity and the demands of long aseptic runs have kept it from becoming the standard. Thomas Falkman, Senior Scientist, describes an approach in which a fixed volumetric feed rate sets the growth rate and metabolic profile, removing the need for cell bleed and daily perfusion rate adjustment. The 50 L study compares this approach against steady-state perfusion and fed-batch on volumetric productivity, space-time yield, total yield, and media use. It also covers metabolite trends, environmental conditions, and filter pressure behavior, along with what the results suggest for automation.

Delve into the data to see how the three strategies compare.