Cytiva product datasheets, catalogs, app notes

51. Evolution Of Large-Scale Chromatography Column Packing 3/16/2016

    Chromatography has always played a crucial role in the purification of biologics. It has constantly evolved, and methods and capabilities have improved to reflect the industry requirements. This article gives an overview of how experience, innovation, and dialogue with customers led to the development of a platform that has revolutionized the economics of large-scale chromatography column packing.

52. Microbial Fermentation In Single-Use Xcellerex™ XDR-50 MO Fermentor System 10/24/2013

    This application note describes the performance of a single-use Xcellerex XDR-50 bioreactor system when used in cultivations of E. coli and of modified Pseudomonas fluorecsens (P. fluorescens) producing a monoclonal antibody (MAb). Both the achieved microbe densities and product yield were shown to be consistent with the performance of conventional stainless steel systems.

53. Obtaining Good Yields With High Purity Of An N-terminal Histidine-Tagged Protein 10/16/2013

    ÄKTA start eliminates the hassles of manual protein purification handling, such as loading large sample volumes and continually monitoring UV readings, and can give you consistency of yield and purity. Madhu demonstrates how ÄKTA start aided him in purifying a recombinant N-terminal histidine-tagged protein.

54. Automating Protein Purification Processes Achieves Reliable Results And Reproducibility 10/16/2013

    ÄKTA start is an easy-to-use, reliable protein purification system. In this application note it is described how Erica, a post-doctoral researcher, used ÄKTA start to purify GST-tagged protein using a predefined method template, a GSTrap FF column, and a Frac30 fraction collector. 

55. Process For Scale-Up Of Adherent Vero Cells From Static Cell Factories To Influenza Production 5/29/2013

    In cell culture-based vaccine production, scale-up of adherent cells is challenging. This study shows a process for scaling up adherent Vero cells from static cell factories to influenza production at 50 L scale using WAVE Bioreactor™ systems and ReadyToProcess single-use equipment.

56. Efficiency Test Of ReadyToProcess™ Columns 5/1/2013

    ReadyToProcess™ columns are prepacked, prequalified, and presanitized process chromatography columns suited for purification of biopharmaceuticals (e.g., proteins, vaccines, plasmids, and viruses) for clinical phase I and II studies. ReadyToProcess columns are available with several media at different volumes (1, 2.5, 10, and 20 L).

57. Propagation Of Influenza Virus In Vero Cells 2/9/2012

    The aim of this work was to establish a process to produce influenza virus in Vero cell culture using single-use equipment. The end result would be a fast and simple process enabling scale-up and adaptation to industrial production. The following parameters were evaluated: medium composition, cell detachment, medium supplements, agitation conditions, and infection conditions. 

58. Two-Step Purification Of Monoclonal IgG1 From CHO Cell Culture Supernatant 4/13/2011

    The increasing demand for monoclonal antibodies (MAb) as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the demand for more effi cient purifi cation processes has increased. This application note describes a two-step process for MAb purifi cation based on MabSelect SuRe™ and Capto™ adhere, a strong multimodal anion exchanger. 

59. Screening Of Loading Conditions On Capto S Using A New High-Throughput Format, PreDictor Plates 2/13/2008

    Finding the optimal conditions for a downstream purification process is of critical importance to gain high productivity and to achieve a robust biopharmaceutical manufacturing process at large scale

60. Automated Cross Flow Filtration For Process Development, Filter Screening, And Process Optimization At Small Scales 9/13/2007

    This application note focuses on microfiltration (pore sizes between 0.1 µm and 1 µm). Microfiltration is ideally suited for separation of cultured cells from the growth medium (broth), as well as for sterilization, and contaminant and particle removal in numerous biopharmaceutical processes