This note compares cell growth and recombinant protein production of multiple Chinese hamster ovary cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplements.
When evaluating media with the goal of increasing titers, it is important to ensure that protein quality is not negatively impacted. So how can this process improvement be performed reliably? It makes sense to use a Design of Experiments (DoE) and empirical approach. Here is an example of how to do it.